Vitamin B2 Reagent – Jaj International

Riboflavin (vitamin B-2) is an important B-vitamin that is found in a large variety of foods . Vitamin B-2 is known to be the central component of the cofactor’s flavin adenine dinucleotide (FAD) and flavin mononucleotide (FMN), which is necessary to all flavoproteins .For this reason, vitamin B-2 is necessary for a large variety of cellular processes . A deficiency of riboflavin has been shown to be associated with poor iron assimilation, an etiology link to anemia, and as a risk for cancer . As intake of riboflavin increases so does the urinary excretion clearance, with a steady maintained excretion increase as the intake continues, having an absorption half-life of 1.1 hours .

Riboflavin is an important participant in numerous diverse internal redox reactions as a part of metabolism and an inadequate intake of this vitamin would contribute to difficulties in intermediary metabolism . A variety of clinical applications for riboflavin administration have been determined; these include (1) large dose intake to resolve infant neurological abnormalities, anemia, and visual impairment  and (2) prevention of migraines at high doses .

Riboflavin deficiency is found to be endemic in many regions of the world and that certain sections of populations in developed countries show a low intake with deficiency endemic in populations having diets lacking dairy products and meat . If intake of riboflavin is poor during maternity status, then the infant is likely to be born riboflavin deficient .

Large-scale surveys conducted within the United States have reported noticeable riboflavin deficiencies among the elderly to be between 10% and 27% . As to athletic performance, various studies have indicated that vigorous exercise can deplete riboflavin . Riboflavin has been studied, with some positive results, to be an attractive candidate for reducing iron in heme proteins for the protection of tissues from oxidative injury .

Considering the substantial nutritional importance of riboflavin, the detrimental effects of deficiency, and potential for beneficial clinical use, then a broad diverse methodology to assay for the vitamin would be very efficacious. Taking advantage of the aqueous solubility of the vitamin, previous work has demonstrated that the vitamin can be estimated in mixtures that are also 0.1 normal in NaOH and with a double-beam ultraviolet-visible spectrometer . Alternatively, a simultaneous determination of B-1, B-2, B-3, and B-6 was accomplished by reversed-phase C-18 column with high-performance liquid chromatography (HPLC).

Fluorescence spectroscopy was successfully applied for vitamin B-2 determination having lesser sensitivity for detection  Utilizing high-performance thin layer chromatography and various mobile phase solvents, such as 1-butanol-chloroform-acetic acid-ammonia-water, 7 : 4 : 5 : 1 : 1, or benzene-methanol-acetone-acetic acid, 70 : 20 : 5 : 5, or chloroform-ethanol-acetone-ammonia, 2 : 2 : 2 : 1, achieved some useful level of separation for riboflavin identification .

Other various liquid chromatography approaches were able to extract vitamins B-1 and B-2 from foods for determination  and use reverse-phase chromatography with 254 nm detection , biosensor technology  ion-pair chromatography, determination in dairy products , foodstuffs , detection of casein , total riboflavin in foods , riboflavin in infant formula  and simultaneous thiamine, riboflavin, and niacin in food . Methods of a riboflavin assay that utilize UV/visible spectrometry with solvation into aqueous media for speed, ease of application, low cost, high sensitivity, accuracy, and extensive application are very few.

The aim of this study is the presentation of a riboflavin assay methodology that has ease of application, high sensitivity, and accurate. Vitamin assay is an important and very large theater within areas of commercial production, food evaluation, and human-assembled merchandise manufacturing. The many types of uses that vitamins are applied to will require a continued investigation for determination of quantity and quality of vitamin presence. Furthermore, the potential benefits of the clinical applications of vitamin therapy require diverse and versatile methodologies for determining these and other vitamins.

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