HBeAb (Anti-HBe) Reagent – Jaj International

Anti-HBe assay is a chemiluminescent microparticle immunoassay (CMIA) for the qualitative detection of antibody to hepatitis B e antigen (anti-HBe) in human serum and plasma and is indicated as an aid in the diagnosis and monitoring of hepatitis B viral infection.

Description

Anti-HBe antibody test is a competitive enzyme immunoassay in which anti-HBe antibodies from specimens compete with a constant amount of Horseradish Peroxidase (HRP) conjugated anti-HBe antibody for a limited number of HBeAg in the neutralizing reagent added to the well. The microtiter well is coated with polyclonal antibodies against HBeAg as catcher for HBeAg.

A serum specimen is added to the microtiter wells together with HRP conjugated anti-HBe-HBeAg complex. After incubation, anti-HBe antibodies in specimen, if present, compete with constant amount of HRP- conjugated anti-HBe for limited amount of HBeAg added into the wells.

The unbound enzyme conjugates will be washed away and the chromogen substrate solution containing hydrogen peroxide is added to the wells for color development. Thus, the amount of HRP-conjugated anti-HBe bound to the well is inversely proportional to the concentration of anti-HBe antibody in the specimen. The absorbance of controls and specimens is determined using EIA reader with wavelength set at 450 nm (450 nm/630 nm).

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