Thyroxine (TT4) Test Kit (Dry Fluorescence Immunoassay)

This TT4 blood test kit is used for the quantitative determination of total triiodothyrine (TT₄) in human serum, plasma, or whole blood in vitro.

Glucocorticoids reduce the levels of TSH and thyroid-binding globulin (TBG) in the blood, so a decrease in serum TT₄ can occur in all causes of hypercortisolism. TT₄ increased significantly in hyperthyroidism, and TT₄ concentration increased in patients with hyperthyroidism T₄, while TT₃ concentration was normal. Hypothyroidism patients were significantly reduced; Peripheral hypothyroidism, due to defects in or after thyroid hormone receptors, causes peripheral tissues to be insensitive to TT₄ and compensatory secretion of TT₄ is increased.

Testing Principle of Total Thyroxine (TT4) Test Kit (Dry Fluorescence Immunoassay)

• This TT4 blood test kit is used for the quantitative determination of TT₄ concentration in human serum, plasma, or whole blood by fluorescence immunoassay competition method.
• The TT₄ antigen in the dissociated sample binds to a fluorescent microsphere labeled mouse anti-human TT₄ monoclonal antibody on the binding pad to form a reaction complex.
• The reaction complex move forward along the nitrate cellulose membrane by chromatography and is not captured by the TT₄ antigen precoated on the test area (T) of the nitrate cellulose membrane, while the excess labeled antibody is captured by the goat anti-mouse IgG polyclonal antibody coated on the NC membrane in the quality control area (C).
• After the reaction is completed, the fluorescence intensity of T line and C line is detected by fluorescence immunoassay quantitative analyzer, and the concentration of TT₄ in the sample is calculated.
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