Colistin – 10 μg Antibiotic Disc – Bioanalyse

The Colistin – 10 μg Antibiotic Disc – Bioanalyse is a paper disc impregnated with 10 micrograms of colistin, a polymyxin antibiotic. It is used in the disc diffusion method of antimicrobial susceptibility testing to determine the susceptibility of bacteria to colistin.

The disc diffusion method is a simple and inexpensive method for testing the susceptibility of bacteria to antibiotics. It is based on the principle that bacteria that are susceptible to an antibiotic will not be able to grow near the disc containing the antibiotic.

To perform the disc diffusion test, a bacterial suspension is spread on the surface of an agar plate. Antibiotic discs are then placed on the surface of the agar plate. The plates are incubated for 16-24 hours, and then the zones of inhibition around the discs are measured. The size of the zone of inhibition is proportional to the susceptibility of the bacteria to the antibiotic.

The Colistin – 10 μg Antibiotic Disc – Bioanalyse is a valuable tool for the diagnosis and treatment of bacterial infections caused by multidrug-resistant bacteria, such as carbapenem-resistant Enterobacteriaceae (CRE) and Pseudomonas aeruginosa.

How to use the Colistin – 10 μg Antibiotic Disc – Bioanalyse:

1. Prepare a bacterial suspension by inoculating a loopful of bacteria into a tube of broth.
2. Incubate the broth tube at 37 degrees Celsius for 2-4 hours, or until the bacteria are in the logarithmic phase of growth.
3. Dilute the bacterial suspension to a turbidity of 0.5 McFarland standard.
4. Spread the bacterial suspension on the surface of an agar plate.
5. Place the Colistin – 10 μg Antibiotic Disc on the surface of the agar plate.
6. Incubate the plate at 37 degrees Celsius for 16-24 hours.
7. Measure the diameter of the zone of inhibition around the disc.
8. Interpret the results using the clinical breakpoints for colistin.

Interpretation of results:

| Zone of inhibition | Interpretation | |—|—|—| | ≥ 10 mm | Susceptible | | 6-9 mm | Intermediate | | < 6 mm | Resistant |

It is important to note that the disc diffusion method is only a semi-quantitative method for testing the susceptibility of bacteria to antibiotics. For more accurate results, other methods such as broth microdilution or agar dilution should be used.

The Colistin – 10 μg Antibiotic Disc – Bioanalyse is a valuable tool for the diagnosis and treatment of bacterial infections caused by multidrug-resistant bacteria. However, it is important to note that colistin is a nephrotoxic antibiotic, which means that it can cause kidney damage. Therefore, colistin should only be used as a last resort for infections caused by multidrug-resistant bacteria.

Limitations of the Colistin – 10 μg Antibiotic Disc – Bioanalyse:

• The disc diffusion method is only a semi-quantitative method for testing the susceptibility of bacteria to antibiotics. For more accurate results, other methods such as broth microdilution or agar dilution should be used.
• The disc diffusion method is not able to detect all bacteria that are resistant to colistin. For example, some strains of Pseudomonas aeruginosa are resistant to colistin, but they may not produce a zone of inhibition around a colistin disc.
• The disc diffusion method can be affected by the type of agar plate used, the incubation temperature, and the inoculum density. Therefore, it is important to follow standardized procedures when performing the disc diffusion test.

Additional information about colistin:

Colistin is a polymyxin antibiotic. It works by disrupting the cell membranes of bacteria. Colistin is generally well-tolerated, but it can cause side effects such as nausea, vomiting, diarrhea, and dizziness. In rare cases, colistin can cause serious side effects such as kidney damage and seizures.

Colistin is a valuable antibiotic for the treatment of bacterial infections caused by multidrug-resistant bacteria. However, it is important to be aware of the potential side effects of colistin before using it.

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