Chloramphenicol – 30 μg Antibiotic Disc – Bioanalyse is a paper disc impregnated with 30 micrograms of chloramphenicol, a broad-spectrum antibiotic. It is used in the disc diffusion method of antimicrobial susceptibility testing to determine the susceptibility of bacteria to chloramphenicol.

The disc diffusion method is a simple and inexpensive method for testing the susceptibility of bacteria to antibiotics. It is based on the principle that bacteria that are susceptible to an antibiotic will not be able to grow near the disc containing the antibiotic.

To perform the disc diffusion test, a bacterial suspension is spread on the surface of an agar plate. Antibiotic discs are then placed on the surface of the agar plate. The plates are incubated for 16-24 hours, and then the zones of inhibition around the discs are measured. The size of the zone of inhibition is proportional to the susceptibility of the bacteria to the antibiotic.

The Chloramphenicol – 30 μg Antibiotic Disc – Bioanalyse is a valuable tool for the diagnosis and treatment of bacterial infections. It can be used to identify bacteria that are susceptible to chloramphenicol, and to determine the optimal dose of chloramphenicol to use for treatment.

How to use the Chloramphenicol – 30 μg Antibiotic Disc – Bioanalyse:

1. Prepare a bacterial suspension by inoculating a loopful of bacteria into a tube of broth.
2. Incubate the broth tube at 37 degrees Celsius for 2-4 hours, or until the bacteria are in the logarithmic phase of growth.
3. Dilute the bacterial suspension to a turbidity of 0.5 McFarland standard.
4. Spread the bacterial suspension on the surface of an agar plate.
5. Place the Chloramphenicol – 30 μg Antibiotic Disc on the surface of the agar plate.
6. Incubate the plate at 37 degrees Celsius for 16-24 hours.
7. Measure the diameter of the zone of inhibition around the disc.
8. Interpret the results using the clinical breakpoints for chloramphenicol.

Interpretation of results:

• Susceptible: Zone of inhibition is greater than or equal to 23 millimeters.
• Intermediate: Zone of inhibition is 17-22 millimeters.
• Resistant: Zone of inhibition is less than 17 millimeters.

It is important to note that the disc diffusion method is only a semi-quantitative method for testing the susceptibility of bacteria to antibiotics. For more accurate results, other methods such as broth microdilution or agar dilution should be used.

The Chloramphenicol – 30 μg Antibiotic Disc – Bioanalyse is a valuable tool for the diagnosis and treatment of a wide range of bacterial infections, including:

• Typhoid fever
• Meningitis
• Rickettsial infections
• Chlamydial infections
• Anaerobic infections
• Skin and soft tissue infections

Limitations of the Chloramphenicol – 30 μg Antibiotic Disc – Bioanalyse:

• The disc diffusion method is only a semi-quantitative method for testing the susceptibility of bacteria to antibiotics. For more accurate results, other methods such as broth microdilution or agar dilution should be used.
• The disc diffusion method is not able to detect all bacteria that are resistant to chloramphenicol. For example, some strains of Enterobacteriaceae are resistant to chloramphenicol, but they may not produce a zone of inhibition around a chloramphenicol disc.
• The disc diffusion method can be affected by the type of agar plate used, the incubation temperature, and the inoculum density. Therefore, it is important to follow standardized procedures when performing the disc diffusion test.

Additional information about chloramphenicol:

Chloramphenicol is a broad-spectrum antibiotic that is effective against a wide range of bacteria. It is typically administered orally or intravenously. Chloramphenicol is generally well-tolerated, but it can cause serious side effects, such as bone marrow suppression and aplastic anemia. Therefore, chloramphenicol should only be used when other antibiotics are not effective or are not tolerated.

Chloramphenicol is a valuable antibiotic that can be used to treat a wide range of bacterial infections. However, it is important to be aware of the potential side effects of chloramphenicol before using it.